Reconstitution Calculator
Calculate the correct draw-up volume for your research compound. Select your syringe, vial, water, and dose.
Reconstitution Guide
Lyophilised (freeze-dried) peptides are delivered as powders that are resistant to short-term temperature fluctuations during transport. At room temperature, peptides in powder form remain stable for several weeks. Proper storage is essential to maintain integrity.
Before use, the lyophilised powder must be reconstituted by mixing it with an appropriate solvent. Accurate reconstitution ensures that peptides retain their potency and bioactivity for research purposes.
- Wash hands thoroughly and wear clean, non-sterile gloves
- Work on a clean, flat surface wiped with 70% isopropyl alcohol
- Have all materials ready: vial, bacteriostatic water, syringe, alcohol swabs
- Ensure the vial is at room temperature before reconstitution
- Wipe the vial rubber stopper with an alcohol swab and let it dry
- Draw the calculated volume of bacteriostatic water into the syringe
- Inject the water slowly down the inside wall of the peptide vial
- Do not shake - gently swirl or roll the vial between your palms until dissolved
- The solution should be clear and colourless. Cloudiness or particulates indicate a problem
- Refrigerate at 2-8C immediately after reconstitution
- Most reconstituted peptides are stable for 28-30 days when refrigerated
- Avoid repeated freeze-thaw cycles - aliquot into single-use quantities if freezing
- Keep away from direct light. Amber vials or opaque containers are preferred
- Use a new sterile needle and syringe for each withdrawal
Peptide Reconstitution FAQ
Add bacteriostatic water (or another sterile diluent) to the lyophilised peptide vial, let it dissolve fully without shaking, then use the calculator above to determine how many syringe units to draw for your target dose.
Bacteriostatic water (0.9% benzyl alcohol) is the standard diluent for multi-dose vials because the preservative inhibits bacterial growth. Sterile water is single-use only. The calculator supports any diluent volume.
Concentration = peptide mass divided by diluent volume. Draw volume = target dose divided by concentration. The calculator converts the result to insulin-syringe units (1 unit = 0.01 mL) for U-100 syringes.
The same reconstitution math applies to any lyophilised product diluted with a known volume. PeptideWatch provides this calculator for educational and research reference only; for medications, follow your prescriber or pharmacist instructions.
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